First Report of Root Rot Caused by <i>Calonectria montana</i> on Sugar Beet in Heilongjiang Province, China

HomePlant DiseaseVol. 105, No. 10First Report of Root Rot Caused by Calonectria montana on Sugar Beet in Heilongjiang Province, China PreviousNext DISEASE NOTE OPENOpen Access licenseFirst ChinaHongtao Shao and Haiying LiHongtao Shaohttps://orcid.org/0000-0003-1607-5608Engineering Research Center Agricultural Microbiology Technology, Ministry Education, University, Harbin 150500, ChinaKey Laboratory Molecular Biology, College School Life Sciences, 150080, ChinaCollege Advanced Agriculture Ecological Environment, Harbin, ChinaSearch for more papers this author Li†Corresponding author: H. Y. Li; E-mail Address: [email protected]://orcid.org/0000-0001-5154-0910Engineering AffiliationsAuthors Affiliations Hongtao Shao1 2 3 Li1 † 1Engineering 2Key 3College Published Online:22 Oct 2021https://doi.org/10.1094/PDIS-10-20-2252-PDNAboutSectionsView articlePDFPDF PlusSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleSugar beet (Beta vulgaris L.) is a crop with economic value northern China, especially Province. In October 2019, root rot was discovered ‘HDW09’ sugar Hulan (126.64° E, 46.00° N), China. Symptoms included lesions tissues, which were initially small dark brown gradually became irregular shapes black. As the disease progressed, necrosis penetrated from external layers into inner tissue. tissues suffered severe decay, resembling several previously reported diseases (Harveson 2006). Pieces transition zones (3 5 mm) between asymptomatic symptomatic surface sterilized 15 s 1% NaClO, rinsed twice distilled water, plated corn meal agar (CMA) supplemented penicillin G (50 mg/liter), incubated at 25 ± 2°C dark. Isolates belonging sp. recovered purified using hyphal tipping technique. Four isolates (A1, A5, A6, A7) used morphological characterization identification DNA sequencing. The 7-day-old colonies malt extract produced buff wooly aerial mycelia. They sienna umber. Chlamydospores microsclerotia abundantly throughout medium. also cultured PDA 25°C under near-ultraviolet irradiation. Macroconidiophores comprised stipe, penicillate arrangement fertile branches, stipe extension, terminal vesicle. Stipe extensions septate, straight flexuous, 61 117 μm long, 4 wide apical septum, terminating sphaeropedunculate vesicle, 9 diameter. Conidiogenous apparatus 31 177 long 16 110 (n = 30). Primary branches conidiogenous aseptate or one-septate, 51 × 7 µm; secondary aseptate, 6 tertiary 8 19 µm, each branch producing one six phialides. Conidia, cylindrical, rounded both ends, straight, 32 53 µm (mean 47 µm), lacking visible abscission scar, held parallel cylindrical clusters colorless slime 100). Partial sequences calmodulin (CAL) (Carbone Kohn 1999), histone H3 (HIS), translation elongation factor 1-alpha (EF1-alpha), beta-tubulin (tub2) (Crous et al. 2004) genes obtained deposited GenBank as MW118652 MW118667. BLAST results showed that CAL, HIS, EF1-alpha, tub2 A1, A7 matched C. strain CERC 8957 MF527082.1 (99 100%), 8930 MF527061.1 (HIS) (98 99%), HSP4 MN356465.1 (EF1-alpha) (100%), MN356460.1 respectively. A phylogenetic tree maximum likelihood algorithm four concatenated reconstructed RAxML revealed clustered clade montana. pathogen identified based molecular traits (Liu 2017; Stępniewska 2020). Ten 8-week-old plants without symptoms selected. roots near ground carefully cleaned. One mycelial plug (5 mm diameter) colony A1 inoculate root. inoculated plugs noncolonized served control. Pathogenicity tests repeated three times. Plants greenhouse conditions watered when soil dry. All like those field after 30 days; control remained healthy. Symptomatic CMA days 25°C. reisolated 100% confirmed sequencing, validating Koch’s postulates. This first report causing beet. study suggests its broader host range wider geographical distribution lays basis further monitoring managing pathogen.The author(s) declare no conflict interest.References:Carbone, I., Kohn, L. M. 1999. Mycologia 91:553. https://doi.org/10.1080/00275514.1999.12061051 Crossref, ISI, Google ScholarCrous, P. W., 2004. Stud. Mycol. 50:415. ScholarHarveson, R. 2006. Plant Health Prog. 7. doi: 10.1094/PHP-2006-0915-01-DG. https://doi.org/10.1094/PHP-2006-0915-01-DG Link, ScholarLiu, Q., 2017. MycoKeys 26:25. https://doi.org/10.3897/mycokeys.26.14688 ScholarStępniewska, H., 2020. For. Pathol. 50:e12595. https://doi.org/10.1111/efp.12595 ScholarThe interest.Funding: work supported National Natural Science Foundation (grant award no. 32072122) Provincial Key Genetic Engineering Biological Fermentation Cold Region.DetailsFiguresLiterature CitedRelated Vol. 10 2021SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionPycnidia Leptosphaeria biglobosa formed necrotic tissue blighted leaf petiole Brassica rapa subsp. pekinensis (C. Yu al.). Photo credit: Yu. Panicle blast rice site sub-Saharan Africa (S. K. Mutiga V. Were. bud soft agave caused Pantoea dispersa (F. Palemón-Alberto S. Ortega-Acosta. Metrics Downloaded 335 times Article History Issue Date: 28 Dec 2021Published: 22 2021First Look: 27 Apr 2021Accepted: 24 2021 Page: 3292 Information© American Phytopathological SocietyFundingNational ChinaGrant/Award Number: 32072122KeywordsCalonectria montanasugar beetpathogen detectionThe interest.Cited ByGenome Sequence Resource annotation montana, an Economically Important Pathogen Broad Host RangeYuee Tian, Yan Li, Shuhe Wang, Jiaxuan He, Di Sun, Shengming Liu, Genqiang Chen, Zhiping Che28 February 2022 | Plant-Microbe Interactions, 0, ja